High resolution melt (HRM) analysis is a relatively new technique used in detecting small variations in DNA sequences between varying populations. Important applications of HRM include SNP analysis, genotyping and methylation analysis. Sean Taylor, Field Application Specialist, Bio-Rad Laboratories, Canada, presented a 20 minute tutorial webinar on high resolution melt analysis and how to use it in research. The video contains information on:

• what is high resolution melt analysis, how does it work and why is it useful
• various applications for high resolution melt analysis including single nucleotide polymorphism (SNP) analysis and DNA methylation analysis
• the difference between a standard melt curve performed in routing QPCR and HRM
• the difference between non-saturation dyes such as SYBR green and saturation dyes such as evagreen
• the definition of the four class types of SNPs
• critical factors for good experimental desighn
• what technique you should be using for your SNP analysis (it is not one size fits all…think HRM vs Sequenome vs multiplex array
• and more!!!