PCR is probably the most important technique in the molecular biologist’s arsenal. Whether it is real-time qPCR or conventional thermal cycling, PCR has provided scientists with a robust research tool that has undergone monumental changes over the past 29 years.
Bio-Rad Laboratories, a leader in PCR tools and research reagents, maintains a website that is geared towards helping researchers troubleshoot and improve upon their PCR experiments. The PCR Doctor has tips and tricks for those performing complicated real-time qPCR experiments:
and scientists with conventional PCR questions.
Be sure to checkout the PCR Doctor for more details.