High resolution melt (HRM) analysis is a great technique used in detecting small variations in DNA sequences between varying populations. Important applications of HRM include SNP analysis, genotyping and methylation analysis. Check out the slide deck below to learn the basics of High Resolution Melt Analysis (HRM), applications, important considerations, assay design and optimization and [...]
Now that the New Years holidays are over and most labs are back into the swing of things, it is time to remind you of your New Years resolution to introduce new, cutting edge tools into your lab in 2012. One such tool that you should definitley become more familiar with is High Resolution Melt [...]
Epigenetics is the study of inherited phenotypic changes caused by mechanisms other than mutations in the underlying DNA sequence. In mammalian cells, most of the chromatin—a complex of DNA, proteins, and histones—exists in a condensed, transcriptionally silent form called heterochromatin. The transcriptionally active form of chromatin is called euchromatin; it exists in a relaxed, less [...]
Designing good qPCR assays can be fun! Learn how to overcome difficult assays, designs and optimization while conforming to the MIQE guidelines. For those that attended one of the qPCR and Epigenetics Scientific Conferences, these slides will be a great refresher. For everyone else-you still have a chance! Checkout this link for details. A practical [...]
We’ve talked a lot about the impact of epigenetic events, such as methylation, on how our genes are read and interpreted by our transcriptional machinery (see DNA has more than four bases! and The book of life: it’s HOW you read it that counts). In that light, we would like to introduce you to the [...]
