By now, all of our readers should be acutely aware of the importance of conforming to the MIQE guidelines when performing quantitative real-time PCR. (If you are not, be sure to read A Tribute to the MIQE Guidelines-Two Years Later and watch the video Applications of MIQE to Real Time Quantitative PCR). For the benefit [...]
Real-time quantitative PCR (qPCR) has become the gold standard for validating DNA microarray data and is routinely used to determine gene expression differences between a wide variety of samples. The exquisite sensitivity of the technology permits the detection of a single copy of a target gene in a sample which has led to qPCR now [...]
Designing good qPCR assays can be fun! Learn how to overcome difficult assays, designs and optimization while conforming to the MIQE guidelines. For those that attended one of the qPCR and Epigenetics Scientific Conferences, these slides will be a great refresher. For everyone else-you still have a chance! Checkout this link for details. A practical [...]
I have often been skeptical of the relevance of claims by PCR and supermix companies that their products are capable of detecting DNA or RNA from a single cell. Although it is technically possible to amplify a signal from as little as a single DNA molecule, I reasoned that the background noise level associated with [...]
We’ve talked a lot about the impact of epigenetic events, such as methylation, on how our genes are read and interpreted by our transcriptional machinery (see DNA has more than four bases! and The book of life: it’s HOW you read it that counts). In that light, we would like to introduce you to the [...]
