In previous posts we discussed the trouble of PCR Inhibitors that plague quantitative PCR reactions and wreak havoc in all disciplines from botany to ancient forensic studies (see Beware the dangers of PCR Inhibitors for more on that topic). PCR inhibition is a frequent problem for people working with complex biological samples such as food, [...]
Bio-Rad Laboratories, Inc. announced it has received the Frost & Sullivan 2012 North American Market Penetration Leadership Award in Quantitative and Digital PCR Instrumentation. This annual award honors the company that has demonstrated excellence by growing market share at the fastest rate in its industry, as measured by revenues or units sold. Frost & Sullivan, [...]
A while back we presented you with a Bio-Rad video on Real Time qPCR Data Analysis. I’ve recently come across another independent qPCR analysis video that focuses on the delta-delta Ct method which I am sharing with you below. Feel free to share your comments, but please note that this video was produced by Kanal [...]
Studies of biomarkers associated with the onset of and progression of cancers have been fraught with poor selectivity and have failed to detect mutant sequences with abundances of less than 1 in 100 wild-type sequences. The QX100 ddPCR platform offers a simple and robust means to increase the precision and sensitivity of TaqMan assays for [...]
Cytogenic studies over the past 50 years have hinted at the impact that copy number variations (CNVs) can have on phenotypic traits and disease susceptibility. Given the high incidence and clinical impact of CNVs, a precise, rapid and cost-effective method is needed for high-throughput validation of candidate CNV associations and for subsequent routing deployment in [...]
