Late last year, Bio-Rad Laboratories acquired QuantaLife and its cutting edge Droplet Digital PCR technology. Below is a video presentation given by Dr. Serge Saxonov, VP Application Development, QuantaLife at the meeting for the American Society for Human Genetics in Washington DC in late 2010. In the video, Dr. Saxonov introduces the third generation of [...]
Some of the technical challenges in qPCR research can be traced back to the reverse transcription step and the synthesis of cDNA for qPCR analysis. Limitations in the maximum amount of template RNA that can be added to an RT reaction make it tougher to detect low-abundant target genes and limits the amount of data [...]
Designing good qPCR assays can be fun! Learn how to overcome difficult assays, designs and optimization while conforming to the MIQE guidelines. For those that attended one of the qPCR and Epigenetics Scientific Conferences, these slides will be a great refresher. For everyone else-you still have a chance! Checkout this link for details. A practical [...]
I have often been skeptical of the relevance of claims by PCR and supermix companies that their products are capable of detecting DNA or RNA from a single cell. Although it is technically possible to amplify a signal from as little as a single DNA molecule, I reasoned that the background noise level associated with [...]
We’ve talked a lot about the impact of epigenetic events, such as methylation, on how our genes are read and interpreted by our transcriptional machinery (see DNA has more than four bases! and The book of life: it’s HOW you read it that counts). In that light, we would like to introduce you to the [...]
