European Biotechnologist

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Browsing Posts tagged real time PCR

At times it can feel that performing good PCR is like voodoo. We can help you to remove that variability to obtain great results all the time by: Delivering innovative and quality reagents and plastics to make the good exceptional Providing dedicated training and support for your experiments and projects Join us on the short [...]

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Your current qPCR instrument has likely given you long and trusted service over many years. However, over time the repeated use of the instrument can lead to reduced accuracy, reproducibility and performance. This not only affects the quality and your confidence in the data, but increases the service costs to support your instrument. Why continue [...]

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Some of the technical challenges in qPCR research can be traced back to the reverse transcription step and the synthesis of cDNA for qPCR analysis. Limitations in the maximum amount of template RNA that can be added to an RT reaction make it tougher to detect low-abundant target genes and limits the amount of data [...]

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Designing good qPCR assays can be fun! Learn how to overcome difficult assays, designs and optimization while conforming to the MIQE guidelines. For those that attended one of the qPCR and Epigenetics Scientific Conferences, these slides will be a great refresher. For everyone else-you still have a chance! Checkout this link for details. A practical [...]

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I have often been skeptical of the relevance of claims by PCR and supermix companies that their products are capable of detecting DNA or RNA from a single cell. Although it is technically possible to amplify a signal from as little as a single DNA molecule, I reasoned that the background noise level associated with [...]

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