Choosing the right membrane is a key factor in successful western blotting. In a recent article published in Biocompare, Bio-Rad’s Thomas Davies and Kenneth Oh, take you through some of the most important factors to consider when deciding which membrane to use in your western blotting assay. Topics include: physical characteristics of PVDF and nitrocellulose [...]
In the latest release of the V3 Western Workflow protocol from Bio-Rad Laboratories, you will learn valuable procedures and tips and tricks for streamlining your Western Blot Protocol and acheiving better results. Some useful tips include: choosing the correct protein standard for molecular weight estimation how to adjust image exposure time to avoid signal saturation [...]
We’ve been talking quite a bit lately about the fundamentals of western blotting and the scientific scandals in which it has played a role. This discussion reminds me of a scathing editorial written in the Journal of Clinical Investigations several years ago titled “All data are not created equal.” In the article, executive editor Ushma [...]
In a soon to be published paper that will appear in Analytical Biochemistry, a team of scientists from the LMU and Federal Office for Radiation Protection in Germany and Bio-Rad Laboratories compared two methods of protein normalisation utilized in western blot analysis. In the first method, known as housekeeping protein (hkp) normalisation, the signal of [...]
Significant or not Significant? That is THE question. And what a question it is. When considered in context with the timeless adage “publish or perish” the hunt for significant data becomes a true test of scientific survival of the fittest and a holy grail for labs with any modicum of academic pride. But just how [...]
