In the latest release of the V3 Western Workflow protocol from Bio-Rad Laboratories, you will learn valuable procedures and tips and tricks for streamlining your Western Blot Protocol and acheiving better results.
Some useful tips include:
- choosing the correct protein standard for molecular weight estimation
- how to adjust image exposure time to avoid signal saturation
- which membranes to use to reduce background signal
- how to perform fluorescent and chemiluminescent immunodetection